November 2013, Volume 16, No.33 Abstracts
Research Progress of EB Virus Associated Gastric Carcinoma
SONG Fei-xue，PEI Xia-xia.Department of Medical Oncology，the Second Hospital of Lanzhou University，Lanzhou 730030，China
【Abstract】 The Epstein-Barr virus(EBV) is closely associated with the occurrence and development of many tumors.Gastric cancer is one of the most common malignant tumor throughout the world.EBV can be detected in about 10% tissues of gastric carcinoma cases throughout the world，which indicate that EBV infection plays an important role in the development of EBV associated gastric carcinomas(EBVaGC).In this paper，we introduce the epidemiology，clinical characteristics，possible pathogenesis，clinical diagnosis，treatment and prognosis of EBVaGC，in order to provide a basis for the diagnosis，treatment and prevention of EBVaGC.
【Key words】 Herpesvirus 4，human；Herpesviridae infections；Stomach neoplasms；Epidemiology；Diagnosis；Therapy
Research Progress of Gene Therapy of Lumbar Disc Degeneration
LI Xiang,LI Xin-zhi.Department of Orthopaedics,Renhe Hospital,China Three Gorges University,Yichang 443001,China
【Abstract】 The treatment of diseases caused by lumbar disc degeneration(LDD)has always been subject to much attention.At present,there are mainly conservative treatment and surgical treatment,but none of them can stop or reverse the process of disc degeneration.In recent years,gene therapy for LDD has been receiving much attention.Gene therapy involves the screening of the target gene and target cell,transfer of the therapeutic gene to the recipient cells using appropriate vector,regulation of the expression of target genes so as to achieve the purpose of the recipient cells synthesizing the RNA and expressing proteins in a continuous fashion.Modifying intradiscal gene expression via gene therapy is perhaps the best method for the lumbar disc degenerative diseases in the near future.
【Key words】 RNA interference;Lumbar disc degeneration;Gene therapy;Target gene;Vectors
Clinical Application of Cardiopulmonary Exercise Testing
NING Liang，SUN Xing-Guo.State Key Laboratory of Cardiovascular Disease，Heart-Lung Functional Testing Center，Fuwai Hospital，National Center for Cardiovascular Diseases，Chinese Academy of Medical Sciences and Peking Union Medical College，Beijing 100037，China
【Abstract】 The cardiopulmonary exercise testing(CPET) is an important and only clinical functional test method.While patients do the loaded exercise，the functions of respiratory，circulatory，metabolic and neurohumoral systems were continuously recorded.CPET was applied and interpreted based on the theoretical system of holistic integrative physiology and medicine in order to evaluate the function and healthy condition of the human body，diagnose disease，grade disease severity，assess therapeutic effect and predict the prognosis.This article studies the CPET in terms of its physiological basis and detection indexes，as well as their significances.The article also discusses the role of CPET in evaluating cardiovascular disease，respiratory disease，disease rehabilitation and therapeutic effect，as well as evaluating the risk of operation with anesthesia and functional status of health.
【Key words】 Cardiopulmonary exercise testing；Diagnosis；Therapy；Rehabilitation；Cardiovascular diseases；Respiratory tract diseases；Holistic integrative physiology
Impacts of Metabolic Syndrome on Pulse Wave Velocity of the Elderly Patients with Primary Hypertension
YANG Hong-xia，MI Shu-hua，JIA Shu-jie，et al.Department of Vip Clinic，Beijing Anzhen Hospital Affiliated to Capital Medical University,Beijing 100029,China
【Abstract】 Objective To explore the impacts of the metabolic syndrome on pulse wave velocity of the elderly patients with primary hypertension.Methods From October 2011 to Febrary 2013,147 elderly patients with primary hypertension were admitted to department of Vip clinic，Beijing Anzhen Hospital,of these patients,80 patients had metabolic syndrome,67 patients had not metabolic syndrome.The height,weight,body mass index(BMI),waist circumference,heart rate,blood pressure,and levels of fasting plasma glucose,TG,TC,HDL-C and LDL-C were measured or calculated. The carotid-femoral pulse wave velocity(C-FPWV) was used to evaluate aortic elasticity using automatic pulse wave velocity(PWV) system.Results There was no statistically significant difference in age,gender,history of primary hypertension,heart rate,systolic blood pressure(SBP),diastolic blood pressure(DBP),TC and LDL-C levels between two groups(P＞0.05).There were statistically significant differences in BMI,waist circumference,fasting blood glucose,TG，HDL-C levels,and C-FPWV(P＜0.05).Waist circumference(r=0.32,P=0.00),fasting plasma glucose level(r=0.12,P=0.03),TG level(r=0.26,P=0.26) were positively correlated with C-FPWV.BMI(r=0.18,P=0.45) and HDL-C level(r=0.09,P=0.09) did not have linear correlation with C-FPWV.Multiple linear regression analysis results showed that the waist circumference has regression relationship with C-FPWV(R2=0.39,P＜0.05).Conclusion C-FPWV is positively correlated with waist circumference,metabolic syndrome can degenerate large artery elasticity in elderly patients with essential hypertension.
【Key words】 Metabolic syndrome；Hypertension；Pulse wave velocity
Post-hemodialysis DN Patients：Occurrence of Pneumonia and Risk Factors Related to Death
ZENG Jian-ying，TONG Jun-rong，ZHANG Jian-lin，et al.Department of Endocrinology，Guangzhou General Hospital of Guangzhou Military Command，Guangzhou 510010，China
【Abstract】 Objective To explore the occurrence of pneumonia and risk factors related to death in post-hemodialysis（HD）patients with diabetic nephropathy（DN）.Methods A total of end-stage renal disease（ESRD）patients hospitalized in this hospital from January 2007 to January 2012 were divided，according whether combined with DN or not，into groups DN（n=190），non-DN（n=955）.After regular HD，the occurrence of pneumonia，death risk factors and survivals were analyzed comparatively.Results The occurrence of pneumonia was higher in DN group than in non-DN group〔12.6%(24/190)与5.6%（54/955）〕，the difference was significant（χ2=12.152，P＜0.05）.By Cox regression analysis，increase of age was an independent death risk factor of pneumonia patients.By Kaplan-Meier cumulative survival curve，the 1-，3-，5-year cumulative all-cause death rates and cumulative pneumonia related death rate were higher in DN group than in non-DN group（P＜0.05）.Conclusion DN patients with HD are more prone to pneumonia，their death risk due to pneumonia is higher than that of non-DN patients with HD.So it is very important that cares for HD patients combined with DN should be strengthened.
【Key words】 Diabetic nephropathy；Renal dialysis；Pneumonia；Risk factors
The Level Change of Serum Leptin and Adiponectin in Patients with Chronic Heart Failure
QIN Yang,XU Tong-tong,LV Xiang-wei.Department of Internal Medicine Teaching and Research,Affiliated Hospital of Guilin Medical College,Guilin 541001,China
【Abstract】 Objective To explore the significance of the levels change of serum leptin and adiponectin before and after treatment in patients with chronic heart failure (CHF).Methods 62 CHF patients admitted to the Department of Cardiac Internal Medicine,VIP,Affiliated Hospital of Guilin Medical College from September 2011 to May 2012 were selected as observation group (17 cases of cardiac function levels Ⅱ,26 cases of level Ⅲ and 19 cases of level Ⅳ).Another 40 healthy people were selected as control group.Body mass index (BMI),blood lipid,blood pressure levels were recorded in the two groups.The plasma leptin,adiponectin,brain natriuretic peptide (BNP),IL-6,LVEF and LVEDd were also detected in the two groups before and after treatment.Results The BMI,TG,TC,HDL-C,LDL-C,systolic pressure and diastolic pressure between control group and observation group showed no statistically significant difference (P＞0.05).In terms of serum leptin,adiponectin,BNP,IL-6 and LVEDd,the patients with cardiac function level Ⅱ,level Ⅲ and level Ⅳ were significantly higher than the control group,and patients of level Ⅲ and level Ⅳ were significantly higher than patients of level Ⅱ,and patients of level Ⅳ were significantly higher than patients of level Ⅲ.When it came to LVEF,the former were significantly lower than the latter ones (P＜0.05).After treatment,the levels of serum leptin,adiponectin,BNP,IL-6 and LVEDd were significantly reduced,but LVEF was significantly increased (P＜0.05).Conclusion The level of serum leptin,adiponectin of patients with CHF is higher than healthy people,serum leptin and adiponectin may be involved in the pathogenesis and development of chronic heart failure,and the monitoring of them may be helpful for the evaluation and treatment of heart failure.
【Key words】 Heart failure；Leptin；Adiponectin
The Association of ApoA5 Gene-1131T＞C and 56C＞G Polymorphism with Type 2 Diabetes Mellitus in Han People of Guizhou
HUANG Jian,HUANG Yun-zhu,YANG Guo-zhen,et al.Department of Biochemical Science,Affiliated Hospital of Guiyang Medical University,Guiyang 550004,China
【Abstract】 Objective To study the association of ApoA5 gene-1131T＞C and 56 C＞G polymorphism with type2 diabetes mellitus (T2DM) and lipid spectrum in Han People of Guizhou.Methods 192 T2DM unrelated patients received by the Affiliated Hospital of Guiyang Medical College during the period from March 2012 to March 2013 were selected as the T2DM group while 206 people with normal level of blood lipid and blood sugar once taking physical examination in our hospital during the same period as the control group.In order to examine the genotype of ApoA5-1131T＞C and 56C＞G,the technology of agarose gel-electrophoresis combined with polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) was adopted.The frequency distribution of the allelic genes was also recorded while the level of blood lipid and blood sugar were examined with automatic chemistry analyzer.Results The distribution of genotype of ApoA5-1131T＞C in the control group (χ2=0.477,P=0.489) and T2DM group (χ2=1.597,P=0.206) has been found to conform to the Hardy-Weinberg law of genetic equilibrium and was also representative of the group.The genotype and allele frequency of the two groups showed statistically significant difference (P＜0.05).The 1131C type carriers (TC+CC) had an 1.965 times increased risk of T2DM compared with TT genotype (OR=1.965) and had a significant increase in TG levels compared with non-C carriers (TT) (P＜0.05).ApoA5 56C＞G was in exon2 of ApoA5,and only CC genotype was detected.Conclusion ApoA5-1131T＞C polymorphism is associated with the risk of T2DM in Han People of Guizhou.C allele has a significantly increased T2DM risk and is associated with the increased serum TG levels.We do not observe ApoA5 gene 56C＞G polymorphism.
【Key words】 Apolipoproteins；Polymorphism,single nucleotide；Diabetes mellitus,type 2
The Relationship between Transient Ischemic Attack and Carotid Plaque and Stenosis Using CT Angiography
LIU Xiao-hong，WU Yu-fu，ZANG Ting-zhen，et al.Department of Neurology，Beijing Geriatric Hospital，Beijing 100095，China
【Abstract】 Objective To discuss the relationship between transient ischemic attack(TIA) and carotid plaque and stenosis using CT angiography(CTA).Methods From 2010-01-01 to 2013-01-01，113 cases with TIA were included in observation group，55 cases without cerebrovascular disease were included in the control group.Multi-director CT angiography(MDCTA) was used to detect carotid plaque and stenosis in all cases，and the severity of carotid plaque，plaque type，plaque distribution in various segments of carotid，extent of carotid stenosis were compared between two groups.Results (1)Both carotid plaque detection rate〔88.5%（100/113）〕 and carotid stenosis detection rate〔70.8%（80/113）〕 in observation group were significantly higher than those〔45.5%（25/55）and 29.1%（16/55）〕 in control group(χ2=43.211，P=0.000；χ2=9.002，P=0.003).Among patients with carotid stenosis in observation group，there were 21 cases(26.2%) of mild stenosis，24 cases(30.0%) of moderate stenosis，22 cases(27.5%) of severe stenosis，13 cases(16.3%) of occlusion.(2)In the observation group，carotid multi vessel plaque was found in 93 cases(82.3%)，in the control group，carotid multi vessel plaque was found in 13 patients(23.6%)，the difference between two groups was statistically significant(χ2=4.218，P=0.045).(3)There was significant difference in the distribution of plaque type between the two groups(P＜0.05)，the plaque type in the control group was dominated by hard plaque(54，accounting for 52.9%)，the plaque type in the observation group was dominated by soft plaque(307，accounting for 42.5%).In the observation group，there was significant difference in the distribution of plaque type(soft plaque，hard plaque，and mixed plaque) in various segments of carotid(P＜0.05).Soft plaque occurred mainly in the common carotid artery(CCA)(152，accounting for 49.6%)，while the hard plaque occurred mainly in the intracranial segment of the internal carotid artery(ICA-I)(129，accounting for 46.9%).Conclusion MDCTA is an important method for the detection and evaluation of carotid plaques and carotid stenosis.Carotid plaque and stenosis detection rates in patients with TIA were higher than those in patients without cerebrovascular disease.The carotid plaque type in patients with TIA was dominated by soft plaque，CCA plaque type was usually soft plaque，which indicated that carotid plaque formation and carotid stenosis are important factors for TIA.
【Key words】 Ischemic attack，transient；Carotid stenosis；Carotid plaque
The Serum Level of Fibulin-5 in Patients with Acute Cerebral Infarction and Its Relationship with Severity of Illness
ZHAO Hui，XU Guang-hui，QIN Xin-yue.Department of Neurology，the First Affiliated Hospital of Chongqing Medical University，Chongqing 400016，China
【Abstract】 Objective To investigate the serum level of serum fibulin-5 in patients with acute cerebral infarction and its relationship with severity of illness.Methods 156 patients with acute cerebral infarction(time of onset＜72 h) who admitted to our department from April to November 2012，were selected as the observation group，and 50 healthy people were selected as control group，the gender and age of control group were similar to those of observation group.The serum levels of fibulin-5 of all cases were detected by ELISA，neurologic impairment scores in observation group were evaluated using NIHSS，the relationships between the serum levels of fibulin-5 and OCSP subtype，infarct size，infarct location and NIHSS score.Results The serum level of fibulin-5 in observation group〔（118.7±51.6）μg/L〕 was significantly higher than that in control group〔（40.1±12.2）μg/L，t=-11.56，P＜0.01〕.The serum level of fibulin-5 in observation group changed with OCSP subtype and infarct size，the differences were statistically significant(F values were 23.48 and 208.32，separately；P＜0.01)；The serum levels of fibulin-5 in patients with total anterior circulation infarction，posterior circulation infarction，partial anterior circulation infarction and lacunar infarction decreased progressively〔（182.1±53.1）μg/L，（135.4±48.7）μg/L，（104.9±21.6）μg/L，（69.3±7.9）μg/L；all P＜0.01〕；There was no significant difference in the serum levels of fibulin-5 among patients with different infarct locations（P＞0.05）.The serum level of fibulin-5 was positively correlated with NIHSS score among patients with acute cerebral infarction（r=0.792，P＜0.01).Conclusion The level of fibulin-5 in patients with acute cerebral infarction was significantly higher than that in healthy people，the level of fibulin-5 in patients with acute cerebral infarction increased with the increased infarct size.However，there was no relationship between fibulin-5 levels and infract locations.The serum level detection of fibulin-5 can offer some valuable guidances to disease assessment for patients with acute cerebral infarction.
【Key words】 Brain infarction；Fibulin-5；Cerebrovascular circulation
The Role of miR-26b in Invasive Ductal Breast Cancer and Its Molecular Mechanism
XU Li-ping,LI Jia,FANG Lin.Department of General Surgery,Ningbo First Hospital,Ningbo 315010，China
【Abstract】 Objective To investigate the expression pattern of miR-26b in breast cancer and the impact of miR-26b on the proliferation of MD-MBA-231 breast cancer cells,and search for its target gene.Methods A total of 38 female patients with breast cancer treated with surgery in the Tenth People′s Hospital of Tongji University in Shanghai from January 2010 to May 2012 were enrolled. The cancer tissue and normal adjacent breast tissue were obtained. Realtime fluorescence quantitative PCR (RT-PCR) was performed to quantify the expression of miR-26b in breast cancer and normal breast specimens. MTT assay was employed to characterize the function of miR-26b in the proliferation of breast cancer cells. The breast cancer cells were treated with 50 nmol/L miR-26b mimic,or 100 nmol/L miR-26b mimic,or miRNA,or liposome,namely the 50 nmol/L miR-26b treatment group,the 100 nmol/L miR-26b treatment group,the negative control group and the blank control group. The proliferation rates in each group were tested after 24 h,48 h,72 h,and 96 h. Target prediction was performed to determine the target gene of miR-26b using the software TargetScan;and the